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KMID : 1138520080110030047
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Journal of Pharmacopuncture
2008 Volume.11 No. 3 p.47 ~ p.53
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The effects of Ganoderma lucidum herba pharmacopuncture on 3T3-L1 preadipocyte differentiation
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Lee Chae-Woo
Youn Hyoun-Min
Kang Kyung-Hwa
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Abstract
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Objective: The purpose of this study is to investigate the effects of Ganoderma lucidum herba pharmacopuncture(GHP) on the adipogenesis in 3T3-L1 preadipocytes.
Methods: 3T3- L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of GHP ranging from 1 and 2%. The effect of GHP on cell proliferation of 3T3-L1 preadipocytes was investigated using MTT assay. The effect of GHP on adipogenesis was examined by Oil red O staining and measuring glycerol-3-phosphate dehydrogenase (GPDH) and intracellular triglyceride (TG) content.
Results: Following results were obtained from the preadipocyte proliferation and adipocyte differentiation of 3T3-L1. We observed no effect of GHP on preadipocyte proliferation. GHP inhibited adipogenesis, the activity of GPDH and accumulation of intracellular TG content.
Conclusions: These results suggest that GHP inhibit differentiation of preadipocyte.
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